. Author manuscript; available in PMC: 2014 Feb 28.

Published in final edited form as: Ann Anat. 2010 Oct 25;193(1):23–29. doi: 10.1016/j.aanat.2010.09.005

Table 1.

Molecular weight of estrogen receptors α and β, androgen receptor and insulin receptor proteins and the specific antibodies used for their detection in Western blot analysis and immunocytochemistry.

Protein	Molecular weight	Method	Antibody	Concentration	Company
ERα	66	WB	Mouse monoclonal anti-ERα	1:500 in 1% BSA	Chemicon, MAB 461
ERα	66	IC	Rabbit polyclonal anti-ERα	1:75 in 1% PBS	Santa Cruz, HC-20, sc-543
ERβ	57	WB	Rabbit polyclonal anti-ERβ	1:500 in 1% BSA	Chemicon, MAB 1410
ERβ	57	WB	Mouse monoclonal anti-ERβ	1:100 in 1% BSA	DAKO, clone 8D5, Dr. J. Askaa
ERβ	57	IC	Rabbit polyclonal anti-ERβ	1:75 in 1% PBS	Bio Reagents, PA1-311
AR	110	WB	Mouse monoclonal anti-AR	1:250 in 1% BSA	DAKO, M3562, clone AR 441^a
AR	110	WB	Rabbit polyclonal anti-AR	1:250 in 1% BSA	Santa Cruz, AR N-20, sc-816^b
AR	110	IC	Mouse monoclonal anti-AR	1:50 in 1% PBS	Lab Vision, AB-1
IR	95	WB	Mouse monoclonal anti-IR	1:200 in 5% SMP	Calbiochem, GR 36, β-subunit
IR	95	IC	Rabbit polyclonal anti-IR	1:50 in 1% PBS	Santa Cruz, C-19, sc-711

Molecular weight is given in kDa. BSA = bovine serum albumin, SMP = skim milk powder, PBS = phosphate buffered saline.

The antibody AR 441 is a monoclonal mouse antibody raised to amino acids 299–315 of AR of human origin.

The antibody N-20 is a rabbit polyclonal antibody raised to a peptide mapping at the N-terminus of AR of human origin.