Table 1.
Protein | Molecular weight | Method | Antibody | Concentration | Company |
---|---|---|---|---|---|
ERα | 66 | WB | Mouse monoclonal anti-ERα | 1:500 in 1% BSA | Chemicon, MAB 461 |
ERα | 66 | IC | Rabbit polyclonal anti-ERα | 1:75 in 1% PBS | Santa Cruz, HC-20, sc-543 |
ERβ | 57 | WB | Rabbit polyclonal anti-ERβ | 1:500 in 1% BSA | Chemicon, MAB 1410 |
ERβ | 57 | WB | Mouse monoclonal anti-ERβ | 1:100 in 1% BSA | DAKO, clone 8D5, Dr. J. Askaa |
ERβ | 57 | IC | Rabbit polyclonal anti-ERβ | 1:75 in 1% PBS | Bio Reagents, PA1-311 |
AR | 110 | WB | Mouse monoclonal anti-AR | 1:250 in 1% BSA | DAKO, M3562, clone AR 441a |
AR | 110 | WB | Rabbit polyclonal anti-AR | 1:250 in 1% BSA | Santa Cruz, AR N-20, sc-816b |
AR | 110 | IC | Mouse monoclonal anti-AR | 1:50 in 1% PBS | Lab Vision, AB-1 |
IR | 95 | WB | Mouse monoclonal anti-IR | 1:200 in 5% SMP | Calbiochem, GR 36, β-subunit |
IR | 95 | IC | Rabbit polyclonal anti-IR | 1:50 in 1% PBS | Santa Cruz, C-19, sc-711 |
Molecular weight is given in kDa. BSA = bovine serum albumin, SMP = skim milk powder, PBS = phosphate buffered saline.
The antibody AR 441 is a monoclonal mouse antibody raised to amino acids 299–315 of AR of human origin.
The antibody N-20 is a rabbit polyclonal antibody raised to a peptide mapping at the N-terminus of AR of human origin.