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. Author manuscript; available in PMC: 2014 Feb 28.
Published in final edited form as: J Biol Chem. 2005 Sep 2;280(46):38544–38555. doi: 10.1074/jbc.M504202200

FIGURE 2. The induction of GADD45β promoter activity by BMP-2 requires signaling by Smad1 interacting with Runx2.

FIGURE 2

A, the pGL2-GADD45β promoter (−1604/+141 bp) was cotransfected with expression vectors encoding the BMP type IB receptor (alk6), Smad1, or Smad6 alone or in combination, or Runx2 or ΔRunx2 alone or in combination. The results show the means ± S.D. of determinations of samples from three or more separate wells. B, the C-28/I2 cells were incubated without (−) or with (+) BMP-2 for 1 h, and total lysates were analyzed on Western blots (WB) using antibodies against phospho-Smad1/5/8 and total Smad1. C, total cell lysates were subjected to immunoprecipitation (IP) with anti-Runx2 and analyzed on Western blots using anti-phosphoserine or Smad1 antibody.