FIGURE 4. Defective mineralization accompanies decreased Mmp-13 mRNA expression in hypertrophic chondrocytes of Gadd45β−/− mouse embryos at 15 dpc.
Tibiae from WT and Gadd45β−/− mouse embryos at 15.5 dpc were examined by toluidine blue staining (A and D), von Kossa staining (B and E), and in situ hybridization for Mmp-13 mRNA (C, F, G, and H). Original magnifications: A–F, ×40; G and H, ×200.