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. Author manuscript; available in PMC: 2014 Feb 28.
Published in final edited form as: J Biol Chem. 2005 Sep 2;280(46):38544–38555. doi: 10.1074/jbc.M504202200

FIGURE 5. Gadd45β−/− mouse embryos at 16. 5 dpc exhibit decreased bone growth associated with decreased levels of Mmp-13 and Col10a1 mRNA in hypertrophic chondrocytes.

FIGURE 5

Whole mounted embryos at 16.5 dpc were stained with Alcian Blue and Alizarin Red to show decreased cartilage matrix and mineralization in ribs (A, arrowheads), forearms (B), and hind limbs (C) of Gadd45β−/− compared with WT mice. Adjacent longitudinal sections from the tibia at 16.5 dpc showed a smaller hypertrophic zone by toluidine blue (T.Blue) staining (D) accompanied by markedly decreased levels of Mmp-13 mRNA (E, arrowhead) and Col10a1 mRNA in Gadd45β−/− mouse embryos compared with WT. Original magnifications: D, ×40; E, ×200.

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