Table 2.
Glycan array analysis of anti-glycan antibodies in human sera from Cryptosporidium sp.-infected individuals. Reactivity of human sera from individuals seropositive for cryptosporidiosis (n = 15), as well as from healthy blood donors (n = 5) were diluted 1:100 and analysed for the presence of anti-glycan IgG antibodies using a Tn glycopeptide array. Three distinct compounds, a peptide with four adjacent GalNAc-threonine residues Ac-PPT*T*T*T*KKP-HN (#13), and two MUC-based peptides, glycopeptides #6 and #7 (displaying two and three adjacent GalNAc-threonines, respectively) were found to have distinct reactivity.
| Mean RFU ±
S.E.M. |
P value | ||
|---|---|---|---|
| G, P samples | N samples | ||
| Peptide #6 | 179 ± 107 | 25.6 ± 3.6 | P < .093 |
| Peptide #7 | 432 ± 235 | 18.60 ± 1.86 | P < .006 |
| Peptide #13 | 1953 ± 1231 | 432 ± 235 | P < .003 |
RFU, relative fluorescent units.