Table 3.

Reactivity (relative fluorescent units; RFU) of pooled human sera from individuals seropositive for cryptosporidiosis, toxoplasmosis and malaria. Pooled serum samples from individuals with known infections of Cryptosporidium sp., Toxoplasma gondii and Plasmodium falciparum were evaluated for reactivity against the Tn glycopeptide array. SD, standard deviation for toxoplasma and plasmodium data.

Peptide	Sequence	Cryptosporidium sera (RFU)	Negative sera (RFU)	Toxoplasma sera (RFU)	SD	Plasmodium sera (RFU)	SD
Cp17_#1	H-ETS*EAAAT*VDLFAFT*LDGGK-NH2	57,119	3,230	27,938	3,312	51,097	4,736
Cp17_#2	H-ETSEAAAT*VDLFAFT*LDGGK-NH2	55,206	2,369	21,454	4,694	38,133	11,834
Cp17_#3	H-ETS*EAAATVDLFAFT*LDGGK-NH2	46,362	974	35,579	2,017	57,692	877
Cp17_#4	H-ETSEAAATVDLFAFTLDGGK-NH2	49,458	1,329	24,072	2,674	56,813	550
Cp17_#5	H-ETS*EAAAT*VDLFAFTLDGGK-NH2	47,425	1,741	4,078	975	7,031	6,967
Cp17_#6	H-ETSEAAAT*VDLFAFTLDGGK-NH2	48,340	2,628	9,086	1,719	16,292	5,424
Cp17_#7	H-ETS*EAAATVDLFAFTLDGGK-NH2	37,957	822	3,524	3,001	2,150	1,126
Cp17_#8	H-ETSEAAATVDLFAFTLDGGK-NH2	14,250	1,112	3,691	1,422	2,402	1,974
Cp17_#9	H-ETT*EAAAS*VDLFAFS*LDGGK-NH2	45,472	2,237	3,675	843	7,391	2,626
Cp17_#10	H-ETTEAAASVDLFAFSLDGGK-NH2	5,239	1,115	2,324	938	2,416	2,319
Cp17	Recombinant Cp17 protein control	60,353	13,384	24,683	3,066	57,535	3,698
Cp23	Recombinant Cp23 protein control	58,873	3,877	39,228	2,031	58,923	2,974
PBS		425	254	350	344	380	743
Tn3-linker	Ac-T*T*T*-CONH2(CH2)3NH2	9,618	477	2,204	1,500	11,346	13,493
Tn-linker	Ac-T*-CONH2(CH2)3NH2	1,532	73	638	300	1,178	1,483
S-GalNAc	C41H72N8O22	123	82	710	145	845	1,965
T-GalNAc	C17H32N4O8	165	188	964	1,037	355	1,063