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. Author manuscript; available in PMC: 2014 Feb 28.
Published in final edited form as: Circ Res. 2013 Aug 15;113(9):1054–1064. doi: 10.1161/CIRCRESAHA.113.302052

Figure 6. Analysis of functional differences in the Raet1e promoter between C57 and FVB.

Figure 6

Based on the results shown in Online Figure XIII, it seems that the major difference in the aortic expression of Raet1e is determined by sequence differences between C57 and FVB in the promoter D fragment. A, Top, Locations of sequence differences between C57 and FVB in the promoter D fragment. Below are the different forms of promoter D analyzed. The black bars indicate C57 sequence, the white bars indicate FVB sequence, and the vertical lines indicate sequence variations introduced by site-directed mutagenesis. The horizontal bar graph indicates the ratio of luciferase/renilla activity for each promoter construct. The black bar indicates the activity of a construct with only C57 sequence, the white bar indicates the activity of a construct with only FVB sequence, and the gray bars designate the activity of modified forms of the Raet1e promoter D. The activity ratio of luciferase/renilla is given as mean±SEM. B, Chromatographic representation of the sequence difference between C57 and FVB in the region of the culprit single nucleotide polymorphism (SNP) rs50817078 (arrow). C, SNP rs50817078 (JMRv10073) localizes to the core of the aortic Raet1e TSS1 based on the sequence obtained for transcript number 1 in Figure 5.