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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 May 2;33(7):1544–1551. doi: 10.1161/ATVBAHA.112.301165

Figure 6. Platelet aggregate formation and stability under shear requires PAK.

Figure 6

(A) Human whole blood was treated with vehicle or PF-3758309 (20 µM) and perfused over collagen at a shear rate of 1500 s-1 to produce platelet aggregates. (B) Quantification of percentage of surface area coverage by platelet aggregates in the presence of vehicle or PF-3758309 (n = 3). (C) Platelet aggregates were formed following perfusion of PPACK-anticoagulated blood over collagen for 4 min at a wall shear rate of 1500 s-1. Following washing for 4 min with buffer containing fibrinogen (3 mg/ml), platelet aggregates were perfused with buffer containing fibrinogen and vehicle (DMSO), IPA-3 (10 µM) or PIR 3.5 (10 µM). Representative DIC images are shown. Scale bar = 100 µm. (D) Quantification of platelet disaggregation promoted by IPA-3 under flow (n = 3). Data are represented as mean ± SEM. Significant results (p < 0.05) are indicated with an asterisk.