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. 2014 Feb 25;15:56. doi: 10.1186/1471-2474-15-56

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Copyright © 2014 Lin et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Long-term effects of HBO on MSCs. (A) HBO significantly increased alkaline phosphatase activity after 7 d (*p < 0.05, n = 4, Student’s t-test), 14 d (**p < 0.01, n = 4, Student’s t-test), and 21 d (**p < 0.01, n = 4, Student’s t-test) of culturing. (B) HBO significantly increased calcium levels after 14 d (*p < 0.05, n = 4, Student’s t-test) and 21 d (**p < 0.01, n = 4, Student’s t-test) of culturing. (C) Greater positive von Kossa staining through the matrix at the surface layer of the HBO group was observed compared to the control group (1.473 ± 0.23-fold, p < 0.05, n = 3, Student’s t-test).