Figure 8. Evaluation of the impact of macrophages and lymphocytes on glucose sensor function in vitro.

Figure 8 represents in vitro cell data to directly determine whether MQ can directly inhibit (bio-foul) sensor in vitro and whether this effect is MQ specific. As can be seen in Figure 8a at 3x10^5 MQ or below (data not shown) did not have any significant impact on either glucose sensor or media glucose levels. Addition of 1x10^6 (Figure 8b) or 3x10^6 (Figure 8b) MQ to the v-well in this in vitro system cause a dose dependent loss of sensor function for the sensor present in the V-wells (blue line in Figure 8a, 8b and 8c). Comparison of sensor function post MQ addition to sensor function pre MQ addition indicated that the MQ did not have any significant impact on sensor function in the absence of the MQ (Figure 8a–8c). Finally, as was the case in vivo, the addition of spleen-derived lymphocytes at comparable numbers did not cause any significant loss of sensor function in this in vitro system (see blue line in Figure 8d).