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Correlation of gene expression by microarray and real-time PCR. RNA was isolated from scraped colonic mucosa. Real-time PCR was done using the ABI 7700 and Taqman Probes. Columns, level of gene expression in colonic mucosa from heterozygous mice relative to wild-type, noninjected mice at baseline by microarray (n = 3) and real-time PCR (n = 4–5). A subset of genes listed in Table 1 were also present on the CodeLink arrays. These genes include Cdh1, Dcn, Esr1, Igf2, Ndr2, and Pik3cg.
