Table 3.
Normalized Expression of Folate Pathway Genes in Hoxd4 and Hoxc8 Transgenic Animals*
|
Hoxd4 |
Hoxc8 |
|||
|---|---|---|---|---|
| Gene | TA/+ +/+ | TA/+ TR/+ | TA only | TA + TR |
| Bhmt | 10.4 ± 0.99 (n = 3) | 10.6 ± 1.61 (n = 8) | 11.1 ± 0.74 (n = 5) | 11.6 ± 1.25 (n = 6) |
| Cbs | 11.41 ± 1.25 (n = 12) | 11.38 ± 0.88 (n = 11) | 11.77 ± 1.11 (n = 27) | 12.50 ± 1.28 (n = 47) |
| Dhfr | 3.01 ± 0.41 (n = 3) | 3.3 ± 0.33 (n = 8) | 3.2 ± 0.35 (n = 5) | 3.23 ± 0.65 (n = 6) |
| Dnmt | 8.19 ± 0.65 (n = 3) | 8.95 ± 0.56 (n = 8) | 8.11 ± 0.47 (n = 5) | 8.48 ± 0.57 (n = 6) |
| Dnmt1 | 6.1 ± 0.49 (n = 6) | 6.62 ± 0.55 (n = 12) | 6.74 ± 2.0 (n = 18) | 6.89 ± 1.88 (n = 28) |
| Dnmt2 | 9.31 ± 0.11 (n = 3) | 9.23 ± 0.48 (n = 8) | 9.55 ± 1.05 (n = 5) | 9.82 ± 0.59 (n = 6) |
| Dnmt3a | 10.46 ± 0.36 (n = 3) | 10.79 ± 0.74 (n = 8) | 10.85 ± 1.54 (n = 13) | 11.45 ± 1.78 (n = 20) |
| Dnmt3b | 10.07 ± 0.27 (n = 3) | 10.07 ± 0.27 (n = 8) | 8.67 ± 0.75 (n = 5) | 9.05 ± 0.48 (n = 6) |
| Folr1 | 13.33 ± 0.78 (n = 4) | 12.73 ± 1.10 (n = 10) | 14.68 ± 1.98 (n = 8) | 13.83 ± 2.54 (n = 17) |
| Folr2 | 12.57 ± 0.85 (n = 7) | 12.75 ± 1.37 (n = 12) | 12.45 ± 1.42 (n = 12) | 12.52 ± 1.74 (n = 17) |
| Folr4 | 9.91 ± 2.24 (n = 7) | 10.68 ± 1.46 (n = 14) | 10.34 ± 1.72 (n = 10) | 9.07 ± 2.59 (n = 12) |
| Mat1a | 10.3 ± 1.42 (n = 3) | 11.35 ± 2.01 (n = 8) | 12.55 ± 0.49 (n = 5) | 12.35 ± 1.03 (n = 6) |
| Mat2a | 3.01 ± 0.46 (n = 12) | 3.18 ± 0.52 (n = 14) | 3.08 ± 0.72 (n = 10) | 3.17 ± 0.64 (n = 18) |
| Ms | 7.04 ± 0.65 (n = 3) | 7.28 ± 0.54 (n = 8) | 6.67 ± 0.83 (n = 5) | 7.24 ± 0.37 (n = 6) |
| Msr | 7.02 ± 0.68 (n = 3) | 7.17 ± 0.39 (n = 8) | 6.72 ± 0.21 (n = 5) | 7.26 ± 0.8 (n = 6) |
| Mthfr | 4.95 ± 0.86 (n = 7) | 5.1 ± 0.39 (n = 12) | 4.44 ± 1.14 (n = 19) | 4.4 ± 1.58 (n = 28) |
| Nat1 | 9.22 ± 1.22 (n = 3) | 9.1 ± 0.89 (n = 8) | 9.08 ± 2.3 (n = 11) | 9.05 ± 1.84 (n = 16) |
| Nat2 | 8.41 ± 0.23 (n = 3) | 8.48 ± 0.4 (n = 8) | 8.95 ± 1.37 (n = 16) | 8.78 ± 1.03 (n = 25) |
| Nat3 | 14.13 ± 1.93 (n = 6) | 14.01 ± 2.13 (n = 12) | 11.97 ± 1.86 (n = 19) | 11.54 ± 2.06 (n = 27) |
| Rfc | 5.48 ± 0.3 (n = 3) | 5.68 ± 0.35 (n = 8) | 6.1 ± 1.74 (n = 8) | 6.07 ± 1.28 (n = 10) |
| Amd1 | 5.19 ± 0.4 (n = 3) | 5.42 ± 0.28 (n = 8) | 5.29 ± 0.63 (n = 5) | 5.48 ± 0.39 (n = 6) |
| Sahh | 3.51 ± 0.42 (n = 3) | 3.88 ± 0.32 (n = 8) | 3.51 ± 0.41 (n = 5) | 3.62 ± 0.31 (n = 6) |
| Tysy | 3.28 ± 0.37 (n = 11) | 3.23 ± 0.37 (n = 14) | 4.09 ± 0.62 (n = 8) | 3.9 ± 0.68 (n = 18) |
| Hoxd4 | 15.2 ± 1.7 (n = 26) | 5.6 ± 0.9 (n = 29) | 13.7 ± 0.83 (n = 14) | 14.5 ± 1.2 (n = 20) |
| Hoxc8 | 2.9 ± 0.61 (n = 11) | 3.0 ± 0.65 (n = 15) | 3.3 ± 1.2 (n = 78) | 0.78 ± 0.94 (n = 85) |
*
ΔCt values were determined separately for all controls (TA only) and TR-containing Hoxd4 and Hoxc8 transgenic animals, and means were calculated for all animals tested in each group (n = from 3 to 85) as indicated. Generally, larger standard deviations were found in cases of lower expression level (high ΔCt value) with correlation coefficients noted where significant at P = .05 (Fig. 5).