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. Author manuscript; available in PMC: 2014 Feb 28.
Published in final edited form as: Birth Defects Res A Clin Mol Teratol. 2006 Apr;76(4):216–229. doi: 10.1002/bdra.20245

Table 3.

Normalized Expression of Folate Pathway Genes in Hoxd4 and Hoxc8 Transgenic Animals*

Hoxd4
Hoxc8
Gene TA/+ +/+ TA/+ TR/+ TA only TA + TR
Bhmt 10.4 ± 0.99 (n = 3) 10.6 ± 1.61 (n = 8) 11.1 ± 0.74 (n = 5) 11.6 ± 1.25 (n = 6)
Cbs 11.41 ± 1.25 (n = 12) 11.38 ± 0.88 (n = 11) 11.77 ± 1.11 (n = 27) 12.50 ± 1.28 (n = 47)
Dhfr 3.01 ± 0.41 (n = 3) 3.3 ± 0.33 (n = 8) 3.2 ± 0.35 (n = 5) 3.23 ± 0.65 (n = 6)
Dnmt 8.19 ± 0.65 (n = 3) 8.95 ± 0.56 (n = 8) 8.11 ± 0.47 (n = 5) 8.48 ± 0.57 (n = 6)
Dnmt1 6.1 ± 0.49 (n = 6) 6.62 ± 0.55 (n = 12) 6.74 ± 2.0 (n = 18) 6.89 ± 1.88 (n = 28)
Dnmt2 9.31 ± 0.11 (n = 3) 9.23 ± 0.48 (n = 8) 9.55 ± 1.05 (n = 5) 9.82 ± 0.59 (n = 6)
Dnmt3a 10.46 ± 0.36 (n = 3) 10.79 ± 0.74 (n = 8) 10.85 ± 1.54 (n = 13) 11.45 ± 1.78 (n = 20)
Dnmt3b 10.07 ± 0.27 (n = 3) 10.07 ± 0.27 (n = 8) 8.67 ± 0.75 (n = 5) 9.05 ± 0.48 (n = 6)
Folr1 13.33 ± 0.78 (n = 4) 12.73 ± 1.10 (n = 10) 14.68 ± 1.98 (n = 8) 13.83 ± 2.54 (n = 17)
Folr2 12.57 ± 0.85 (n = 7) 12.75 ± 1.37 (n = 12) 12.45 ± 1.42 (n = 12) 12.52 ± 1.74 (n = 17)
Folr4 9.91 ± 2.24 (n = 7) 10.68 ± 1.46 (n = 14) 10.34 ± 1.72 (n = 10) 9.07 ± 2.59 (n = 12)
Mat1a 10.3 ± 1.42 (n = 3) 11.35 ± 2.01 (n = 8) 12.55 ± 0.49 (n = 5) 12.35 ± 1.03 (n = 6)
Mat2a 3.01 ± 0.46 (n = 12) 3.18 ± 0.52 (n = 14) 3.08 ± 0.72 (n = 10) 3.17 ± 0.64 (n = 18)
Ms 7.04 ± 0.65 (n = 3) 7.28 ± 0.54 (n = 8) 6.67 ± 0.83 (n = 5) 7.24 ± 0.37 (n = 6)
Msr 7.02 ± 0.68 (n = 3) 7.17 ± 0.39 (n = 8) 6.72 ± 0.21 (n = 5) 7.26 ± 0.8 (n = 6)
Mthfr 4.95 ± 0.86 (n = 7) 5.1 ± 0.39 (n = 12) 4.44 ± 1.14 (n = 19) 4.4 ± 1.58 (n = 28)
Nat1 9.22 ± 1.22 (n = 3) 9.1 ± 0.89 (n = 8) 9.08 ± 2.3 (n = 11) 9.05 ± 1.84 (n = 16)
Nat2 8.41 ± 0.23 (n = 3) 8.48 ± 0.4 (n = 8) 8.95 ± 1.37 (n = 16) 8.78 ± 1.03 (n = 25)
Nat3 14.13 ± 1.93 (n = 6) 14.01 ± 2.13 (n = 12) 11.97 ± 1.86 (n = 19) 11.54 ± 2.06 (n = 27)
Rfc 5.48 ± 0.3 (n = 3) 5.68 ± 0.35 (n = 8) 6.1 ± 1.74 (n = 8) 6.07 ± 1.28 (n = 10)
Amd1 5.19 ± 0.4 (n = 3) 5.42 ± 0.28 (n = 8) 5.29 ± 0.63 (n = 5) 5.48 ± 0.39 (n = 6)
Sahh 3.51 ± 0.42 (n = 3) 3.88 ± 0.32 (n = 8) 3.51 ± 0.41 (n = 5) 3.62 ± 0.31 (n = 6)
Tysy 3.28 ± 0.37 (n = 11) 3.23 ± 0.37 (n = 14) 4.09 ± 0.62 (n = 8) 3.9 ± 0.68 (n = 18)
Hoxd4 15.2 ± 1.7 (n = 26) 5.6 ± 0.9 (n = 29) 13.7 ± 0.83 (n = 14) 14.5 ± 1.2 (n = 20)
Hoxc8 2.9 ± 0.61 (n = 11) 3.0 ± 0.65 (n = 15) 3.3 ± 1.2 (n = 78) 0.78 ± 0.94 (n = 85)
*

ΔCt values were determined separately for all controls (TA only) and TR-containing Hoxd4 and Hoxc8 transgenic animals, and means were calculated for all animals tested in each group (n = from 3 to 85) as indicated. Generally, larger standard deviations were found in cases of lower expression level (high ΔCt value) with correlation coefficients noted where significant at P = .05 (Fig. 5).