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. 2010 Feb 5;31(2):211–218. doi: 10.1038/aps.2009.184

Figure 3.

Figure 3

Effects of 1,10-phenanthroline and CRM197 on the 14,15-EET-induced tyrosine phosphorylation of EGF-R and ERK1/2 in Tca-8113 cells. Serum-deprived Tca-8113 cells were incubated with or without 100 μmol/L of 1,10-phenanthroline or 10 μg/mL of CRM197 for 60 min. They were then stimulated for 30 min with 250 nmol/L 14,15-EET or 20 ng/mL EGF. Phosphorylated EGF-R, total EGF-R, phosphorylated ERK1/2, and total ERK1/2 were detected with the corresponding antibodies. (A) Results of incubation with 1,10-phenanthroline. The results shown are representative of three separate experiments with similar results. (B) Densitometric quantification of three separate experiments examining the effects of incubation with 1,10-phenanthroline. (C) Results of incubation with CRM197. The results shown are representative of three separate experiments with similar results. (D) Densitometric quantification of three separate experiments examining the effects of incubation with CRM197. bP<0.05 vs vehicle control group; eP<0.05 vs no signaling pathway inhibitor group.