Table 3.

Definition of parameters for oocyte propylene glycol addition

Published and defined parametersa
Parameter	Name	Value (at 22 °C)
Lp	Hydraulic Conductivity b	0.53 μm min−1 atm−1
Ps	Solute permeability	16.68 μm min−1
Vi	Initial volume in isosmotic media	2,650,000 μm3
Vb	Osmotically Inactive Volume	503,500 μm3
$V_w^i$	Isosmotic water volume ( $V_w^i=V^i-V_b$)	2,146,500 μm3
$V_w^f$	Desired final water volume	2,146,500 μm3
A	Cell surface area based on sphere of volume Vi	92,539 μm2
V*	Lower volume constraint	0.7 × Vi
T	Temperature	295.15 K
R	Gas Constant	0.08205 L atm K−1 mol−1
Miso	Isosmotic molarity	0.29 mol/L
$c_s^i$	initial intracellular permeating solute concentration	0 mol/L
$c_s^f$	final intracellular permeating solute concentration	3 mol/L

Calculated (unitless) parameters
Parameter	Equation	Calculated value
b	Ps/LpRTMiso	4.48
$x_1^i$	$V_w^i/V_w^i$	1
$x_2^i$	$x_1^i{c}_s^i/M^{\mathit{iso}}$	0
$x_1^f$	$V_w^f/V_w^i$	1
$x_2^f$	$x_1^f{c}_s^f/M^{\mathit{iso}}$	10.34
k*	$V_{\ast }-V_b/V_w^i$	0.51

^aAll values from [29] unless noted.

^bWater and solute permeability values published in the literature were determined using a different flux model. To account for this, the conversion was made in a similar manner to that described in [8].