Figure 7. Histology of tumors treated with CuDox-LTSLs.

a) H&E of NDL tumors treated with CuDox-LTSLs with and without ultrasound post 16 (i, upper and middle images) and 28 days of treatment (ii-iv upper and middles images) compared to control at 16 days post intravenous injection of saline (i, lower image). For treatment CuDox-LTSLs combined with ultrasound, tumors were insonified at 42°C for 5 min prior to and 20 min post injection. b) H&E images of the whole tumor sections (upper panels) and the magnified views enclosed by black boxes (middle panels) post drug release by US at the early (i), middle (ii), and end of the 28-day therapy (iii). Additional sections of tumor (ii) were stained with rhodanine (iv), anti-mouse F4/80 (v), and Prussian blue (vi) to identify copper, macrophages, and iron, respectively. The scale bars correspond to 1 mm (a and upper panels of b) and 100 μm (middle and lower panels of b).