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. 2014 Feb 28;9(2):e83728. doi: 10.1371/journal.pone.0083728

Figure 1. Infection of Huh7.5JFH1 cells with HIV results in increased HCV RNA synthesis.

Figure 1

100,000 Huh7.5JFH1 cells were incubated with DNase-treated HIVNL4-3 (white bars) or HIVYK-JRCSF (grey bars) at an MOI = 1 for two hours. At days 1, 3, 7, and 14 post-infection, positive-sense HCV RNA (A) and negative-sense HCV RNA (B) were quantified in cell lysates, compared to the no HIV condition (black bars) by real-time, strand-specific PCR, and normalized to cellular GAPDH levels as described previously [33]. * p≤0.05; ** p≤0.10 compared to HIV-uninfected Huh7.5JFH1 cells at each time point.