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. 2014 Feb 28;9(2):e83728. doi: 10.1371/journal.pone.0083728

Figure 4. Infection of Huh7.5JFH1 cells with HIV results in increased HCV NS3 and NS5A protein production.

Figure 4

100,000 Huh7.5JFH1 cells were incubated with DNase-treated HIVNL4-3 at an MOI = 1 for two hours. Cell lysates were harvested at days 1, 3, 7, and 14 post-infection for the detection of HCV NS3 and NS5A proteins by Western Blot. Equivalent total cell protein concentrations were loaded, and all data were normalized to cellular GAPDH levels at each time point. The uninfected (no HIV) condition, as well as infection with HIVNL4-3 in the presence of 100 uM AZT, are shown as additional controls.