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. 2014 Feb 28;9(2):e86910. doi: 10.1371/journal.pone.0086910

Table 2. Adhesion of SCLC cells to ECM constituents.

ECM component BME FIB LAM THR PO
Cell line
GLC-2 ++ +++ +++ +++ +++
GLC-36 +++ +++ ++ ++++ +++
H146 + + ++ ++ ++
H1184 +++ +++ +++ ++ ++
SCLC-24H + - +++ +++ +++

ECM constituents or PO were immobilized on plastic surfaces and offered as substrates. Protein concentrations used for coating were 20 µg/ml for BME, FIB and LAM, 40 µg/ml for THR and 50 µg/ml for PO. On a total area of 500 µm2 the subarea covered with adherent cells was estimated. Values are given semiquantitatively: "−" less then 10, "+" 11–30, "++" 31–60; "+++," 61–80 and "++++" 80–100% of the total area were covered with adherent cells.