Figure 4. Tempol blocks parasite growth in vitro.

Comparison with other antioxidants. A, Tempol; B, PBN; C, MnTE-2-PyP; D, MnTBAP; E, Mitotempo; F, Mitoquinone; G, M30; and H, Epigallocatechin gallate were added at the indicated concentrations directly to P. falciparum cultures containing trophozoite-stage parasites. After 48 hours of culture, the respective number of trophozoites present in the cultures was determined by pLDH (growth inhibition assay). Stock solution for all reagents was diluted in the culture assay media, or as specified in Materials and Methods. The chemical structure of each compound, and the corresponding IC_50s, are shown as an inset.