Figure 7. Tempol inhibits increase of permeability of the blood brain barrier (BBB).

Plasmodium berghei Anka parasitized red blood cells (10⁶) were injected into mice intraperitoneally (i.p.) Tempol (100 µL; 20 mg/kg), was started at day 1 or day 4 post infection (p.i.). At day 6 p.i., all animals were euthanized and the brain immediately dissected and fixed. Permeability of the BBB was detected as extravasation of IgG as described in Materials and Methods. A, A typical experiment is shown: i, uninfected mice; ii, infected and treated with Tempol (day 4); iii, infected and treated with Tempol (day 1); and iv, infected, non-treated mice. B, Quantification of inflammation of the whole brain. C, Quantification of inflammation of the olfactory bulbs. Quantification was performed as described in Materials and Methods. Ten mice were analyzed per group. *, P≤0.05 (ANOVA, Tukey post-test).