Table 2. Kinetic parameters of DadB and other HLDs.

Substrates	HLD	K m(mM)	k cat(s−1)	k cat/K m(mM−1•s−1)	k i(mM)
1-Chlorobutane	DbeAa	3.23	0.17	0.05
	DhaAa	0.4	0.86	2.15
	DhlAa	2.2	1.5	0.68
	DmbAa	1.56	0.6	0.38
	DmbAb	0.16±0.04	0.08±0.004	0.5
	DbjAc	4.0±1.8	1.4±0.42	0.35
	LinBd	0.18±0.02	1.6±0.06	8.8
	LinBe	0.23±0.04	1.11±0.05	4.83
	DadB	4.88±0.35	1.17±0.10	0.24
1,3-Dibromopropane	LinBb	24.1±3.23	40.9±5.2	1.7	0.49±0.06
	DmbAb	4.52±0.71	9.20±1.17	2.03	2.65±0.49
	DbjAc	0.22±0.07	3.6±0.49	16
	DadB	0.82±0.04	13.39±0.99	16.43
1,2-Dibromoethane	LinBe	5.54±0.49	29.33±1.19	5.29
	DadB	8.70±0.44	17.95±0.50	2.06
4-bromobutanenitrile	DadB	8.47±1.27	30.80±2.49	3.64	17.93±1.97
3-chloro-2-methylprop-1-ene	DbjAc	0.47±0.29	3.5±1.1	7.4
	DadB	11.33±0.99	34.26±1.99	3.02
2,3-dichloroprop-1-ene	DadB	15.06±1.37	20.06±1.09	1.33

^aChovancova, 2011(Doctoral dissertation).

^bJesenska et al., 2005.

^cSato et al., 2005.

^dNagata et al., 1999.

^eChovancova, 2003.