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. 2014 Feb 28;9(2):e89160. doi: 10.1371/journal.pone.0089160

Figure 2. Homozygous PREPL−/− mice lack PREPL and have diminished Prepl mRNA levels.

Figure 2

A) Western blot for PREPL in the spinal cords of PREPL+/+ and PREPL−/− mice. Recombinant PREPL (lane 1) is used as a positive control to test the polyclonal antibody used to in this blot. Analysis of spinal cord samples from PREPL−/− (lanes 2–4) and PREPL+/+ (lanes 5–7) demonstrate the PREPL−/− mice lack full-length PREPL protein. Removal of exon 11 could result in a truncated PREPL protein, but no evidence of a shorter protein is evident in the western blot of PREPL−/− samples (lanes 2–4). B) Analysis of Prepl mRNA by qPCR in mouse liver reveals that there is substantially less Prepl transcript in the liver of PREPL−/− mice when compared to PREPL+/+, indicating that removal of exon 11 also impacts mRNA levels. (Error bars depict SEM. Statistical significance calculated by a Student's t-test, p-value<0.05, *, N = 4).