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. 2014 Feb 28;9(2):e89188. doi: 10.1371/journal.pone.0089188

Figure 1. RFLP pattern of the ITS region under the restriction enzymes (HhaI and HinfI).

Figure 1

Restriction with HhaI produced two fragments of 550 bp and 430 bp in Hybrid genotype and A. simplex s. s.. HinfI produced four fragments of 620 bp, 370 bp, 300 bp, and 250 bp in Hybrid genotype, whereas produced two fragments of 620 bp and 250 bp in A. simplex s. s.. Lanes: 1, Hybrid genotype; 2, A. simplex s. s.; L, 100-bp ladder.