Figure 3. Comparison of 18S rRNA molecules lacking the 2′-O-ribose methyl group at nucleotide A100 in polysomal RNA and total RNA.

Polysomal RNA and total RNA isolated from wild type cells that were grown in YEPD medium to exponential phase was incubated with or without the DNAzyme 10–23-snR51-A100 and afterwards analyzed by gel analysis. The RNA band marked with an asterisk represents the large 18S rRNA fragment after cleavage at nucleotide A100.