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. 2014 Feb 28;9(2):e89640. doi: 10.1371/journal.pone.0089640

Figure 4. Investigation of changes in Am100 modification extent in 18S rRNA after introduction of a multicopy plasmid containing SNR51.

Figure 4

Total RNA isolated from wild type and Δsnr51 cells grown in synthetic medium to exponential phase containing no plasmid, the plasmid pRS426 or the plasmid pRS426-Cluster3 was incubated with the DNAzyme 10–23-snR51-A100 and afterwards analyzed by gel analysis. The RNA band marked with an asterisk represents the large 18S rRNA fragment after cleavage at nucleotide A100.