Table 2. Analysis of putative CRP-recognition sequences in 5'-UTRs of cAMP-regulon of Mtb.
Locus (Rv/annotation) | Sequencea | Position from Start codon |
Rv0146 | tgtcgaggctttcacc | –325 |
Rv0146 | tttcaccatgaacaca | –316 |
Rv0146 | tgacaccggcatcacg | –45 |
Rv0146 | agcgactcggtttaga | –266 |
Rv0146 | tgtccaggcgttgacc | –244 |
Rv0146 | cgagaccgtccgcacc | –103 |
Rv0264c | gctgatctggatgacc | –300 |
Rv0350/dnaK | agcgaccgtcagcacg | –301 |
Rv0350/dnaK | cgttagcatgctcagt | –135 |
Rv0677c/mmpS5 | tttcactgtactctga | –81 |
Rv0677c/mmpS5 | tgttcgacgaattcca | –149 |
Rv0677c/mmpS5 | tctgaaatctgtgacg | –70 |
Rv1057 | cgtgacctaggtaaca | –248 |
Rv1057 | tcagaatttggtcgct | –208 |
Rv1330c | tgcggaccgcgtcgga | –21 |
Rv1330c | agtcacgtagctcatc | –233 |
Rv1330c | tggcatgcggctcgct | –276 |
Rv2025c | tctgagcaagctcagc | –246 |
Rv2025c | tgcgtatgaatgcaga | –82 |
Rv2590/fadD9 | agtgaggggctggaca | –206 |
Rv2590/fadD9 | cgtcatcattttgacc | –285 |
Rv2590/fadD9 | cgtgccgcatctcaca | –133 |
Rv3830c | tgcgcgcagtctcgcc | –72 |
Rv3830c | tgtcaatgttgacaga | –42 |
Rv3830c | tttgtcaatgttgaca | –44 |
Three hundred fifty base pair long DNA sequences upstream to the respective ORFs were analyzed to identify CRPM-recognition sequence. aThe underlined sequences represent putative CRPM-recognition sequences (TGTGA-N6-TCACA, with two mismatches in any of the arms) in the 5'-UTR of the respective genes.