Figure 5. The influence of QKI-5 overexpression or silencing in PC3 cells and DU145 cells. (A) The effect of QKI-5 overexpression on the G₁ phase and the apoptosis regulators as analyzed by western blot. Western blot analysis showed that in the QKI-5 overexpressing PC3 cells, the cyclin D1 and CDK4 expression levels were downregulated and that the P27 and PARP expression level was upregulated. (B) The effect of QKI-5 silencing on the G₁ phase and the apoptosis regulators as analyzed by western blot. Western blot analysis showed that in the QKI-5 silencing DU145 cells, the cyclin D1, and CDK4 expression levels were upregulated and that the P27 and PARP expression level was downregulated. The relative quantification of QKI-5 and other regulatory protein expression was normalized to the GAPDH protein expression levels. Western blots were analyzed with the ImageJ software. The trend of each group is indicated. The data are presented as the mean ± SD and one-way ANOVA analysis for three independent experiments. *P < 0.05.