Figure 2. Cell-cycle assay was performed on each of the three gastric cancer cell lines (MKN74, MKN45, and NUGC4) following incubation for 96 h with or without irradiation to maintain constant cell culture conditions (representative data was shown in [A]). Three cell-cycle segments are shown in order from left to right: G₁/G₀ phase, S phase, G₂/M phase, and the percentage of cells in each phase is shown in (B). Continuous low-dose-rate irradiation by I-125 seeds induced a lower percentage of G₀/G₁, and higher percentage of G₂/M phase cell-cycle arrest compared with the control for all 3 gastric cancer cell lines (P < 0.05). Experiments were performed at least 3 times.