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. 2014 Feb 28;9(2):e90027. doi: 10.1371/journal.pone.0090027

Table 1. Accession number (a), Gene Symbol (b), primers sequence (c), amplicon length (d) and Tm (e) of transcripts validated in qPCR experiments.

a b c d e
NM_001035325 ANXA3 F 5′ GACCCCACCGGCAGTGTTCG 3′ R 5′ GGCATGGCCGATCTCCTGCAT 3′ 130 bp 64°
NM_174137 SERPINE1 F 5′ GTTTAGGCCGAGCCAGGCGG 3′ R 5′ TGGGATTGTGCCGCACCACG 3′ 206 bp 64°
NM_001079629 TSPAN8 F 5′ CCTGGGATGCTGTGGTGCCAT 3′ R 5′ ACGATACCTGCCGCCACCTGC 3′ 102 bp 64°
NM_001192095 KRT18 F 5′ GCAGACCGCTGAGATAGGAG 3′ R 5′ CAAGCTGGCCTTCAGATTTC 3′ 109 bp 60°
NM_001077864 STX7 F 5′ ACAGAGGACGACCTGCGCCTT 3′ R 5′ TGCGCTGATAGTCTGCCGCC 3′ 217 bp 64°
NM_173908 DDO F5′TGTGAAGGCCCTGCCTACCTCC 3′ R5′ CGGGTGAAGCTCCCACAGGTC 3′ 102 bp 64°
NM_001101065 MIOX F 5′ CTACACGTCTGGCCCGCTCCT 3′ R 5′ CCCCGAACTGGGCATGCTTCC 3′ 104 bp 64°
NM_001166616 C5 F 5′CGCAAACGCAGATGACACCCG 3′ R 5′ TGGGGCCTGCCTGAATCCGA 3′ 200 bp 62°
NM_001075923 ENPP3 F 5′ TGTTGGTGGCTGTGTCACTT 3′ R 5′GGCAGCCCTCTAGTCCTCTA 3′ 117 bp 60°
NM_001192214 DPYS F 5′ CCTTCAACGCCTGACTTCCT 3′ R 5′ AGAGCTTTCTGGCAGCTGTT 3′ 98 bp 58°
GU324291 GAPDH F 5′ TCACTGGCATGGCCTTCCGC3′ R 5′ GCCCTCTGACGCCTGCTTCAC 3′ 122 bp 60°