Table 1. Accession number (a), Gene Symbol (b), primers sequence (c), amplicon length (d) and Tm (e) of transcripts validated in qPCR experiments.
a | b | c | d | e |
NM_001035325 | ANXA3 | F 5′ GACCCCACCGGCAGTGTTCG 3′ R 5′ GGCATGGCCGATCTCCTGCAT 3′ | 130 bp | 64° |
NM_174137 | SERPINE1 | F 5′ GTTTAGGCCGAGCCAGGCGG 3′ R 5′ TGGGATTGTGCCGCACCACG 3′ | 206 bp | 64° |
NM_001079629 | TSPAN8 | F 5′ CCTGGGATGCTGTGGTGCCAT 3′ R 5′ ACGATACCTGCCGCCACCTGC 3′ | 102 bp | 64° |
NM_001192095 | KRT18 | F 5′ GCAGACCGCTGAGATAGGAG 3′ R 5′ CAAGCTGGCCTTCAGATTTC 3′ | 109 bp | 60° |
NM_001077864 | STX7 | F 5′ ACAGAGGACGACCTGCGCCTT 3′ R 5′ TGCGCTGATAGTCTGCCGCC 3′ | 217 bp | 64° |
NM_173908 | DDO | F5′TGTGAAGGCCCTGCCTACCTCC 3′ R5′ CGGGTGAAGCTCCCACAGGTC 3′ | 102 bp | 64° |
NM_001101065 | MIOX | F 5′ CTACACGTCTGGCCCGCTCCT 3′ R 5′ CCCCGAACTGGGCATGCTTCC 3′ | 104 bp | 64° |
NM_001166616 | C5 | F 5′CGCAAACGCAGATGACACCCG 3′ R 5′ TGGGGCCTGCCTGAATCCGA 3′ | 200 bp | 62° |
NM_001075923 | ENPP3 | F 5′ TGTTGGTGGCTGTGTCACTT 3′ R 5′GGCAGCCCTCTAGTCCTCTA 3′ | 117 bp | 60° |
NM_001192214 | DPYS | F 5′ CCTTCAACGCCTGACTTCCT 3′ R 5′ AGAGCTTTCTGGCAGCTGTT 3′ | 98 bp | 58° |
GU324291 | GAPDH | F 5′ TCACTGGCATGGCCTTCCGC3′ R 5′ GCCCTCTGACGCCTGCTTCAC 3′ | 122 bp | 60° |