Figure 2.

The glucose responsiveness of SRC-1 is reversible and is not dependent on ROS but is dependent on nutrient availability. a, A549 cells were grown in the absence or presence of glucose. These cells were then split and grown for 4 passages in the corresponding medium. b, A549 cells passaged without glucose were subsequently changed into medium in the absence or presence of glucose. c, A549 cells were grown in the absence or presence of glucose, with a ROS inhibitor N-acetylcysteine (NAC) or a vehicle control. d, A549 cells were grown under the indicated metabolite treatment. Where indicated, cells were treated with 4.5 g/L glucose, 110 mg/L pyruvate, 4.5 g/L fructose, or 50 mM 2-DG (2-deoxyglucose) for 24 hours. Following all treatments whole-cell lysates were then subjected to immunoblot analysis for the indicated proteins.