Figure 3. Validation of point mutations in the LNCaP and C4-2B cell lines.

Validation was performed using PCR on both genomic DNA and copy-DNA, followed by conventional Sanger sequencing. The first and second column represents the name of the gene and the amino acid substitution respectively. The third, fourth, seventh and eighth column represent next-generation sequencing results for whole exome and transcriptome sequencing, which are then validated with Sanger sequencing in the fifth, sixth, ninth and tenth column. A + denotes that the mutation was detected, a – denotes that the mutation was not detected, while 0 means that no PCR-product could be amplified and/that this position was not covered with next-generation sequencing.