Figure 1. The effect of Cu^II(gtsm) on MTT reduction, LDH release and Cu levels.

The effects of CuCl₂, (gtsm)H₂, Cu^II(atsm) and Cu^II(gtsm) (25 and 50 nM) on NGF-treated PC12 cells was assessed. (A) The treatments used did not affect MTT reduction with the exception of 25 and 50 nM Cu^II(gtsm) that inhibited MTT reduction slightly (n = 4/treatment). (B) LDH analysis of Cu^II(gtsm)-treated cell cultures indicates that concentrations of up to 100 nM can be used with no significant increase in LDH release (n = 4/treatment). (C) ICP-MS results showed that 1 hr exposure to 50 nM Cu^II(gtsm) increased cellular Cu content significantly and at 18 hr produced a significantly higher increase in Cu content than at 1 hr (n = 6/treatment). Values are mean ± SEM. *p<0.05, **p<0.01, ***p<0.001.