Figure 3. Cu^II(gtsm) effects on neurite elongation of NGF-treated PC12 cells.

(A) The effect of CuCl₂, (gtsm)H₂, Cu^II(atsm) and Cu^II(gtsm) (25 and 50 nM, 18 hr) on neurite elongation (assessed as % neurites 2x or more than cell body width). Cu^II(gtsm) induced a significant increase in neurite elongation (at both concentrations used) whereas the other treatments had no effect (n = 3/treatment). (B) The effect of 50 nM Cu^II(gtsm) on neurite elongation was examined further by grouping neurites according to length (measured in µm). Cu^II(gtsm) induced a significant increase in the number of neurites in the 75–99 µm range. Values are mean ± SEM. *p<0.05, **p<0.01.