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. 2014 Feb 28;9(2):e90093. doi: 10.1371/journal.pone.0090093

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© 2014 Piro et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Inline graphic — (A) Full length Glp1r transcript expression as analysed by RT-PCR in α-TC1-6 and in InR1G9 cells. (B) Glp1r gene expression as analysed by Real Time PCR (means of five experiments) in beta cells (β-TC1 cells as positive control) and alpha cells (TC1-6); data are expressed as (2^−ΔΔCt) considering as 1 the Glp1r gene expression in alpha cells (TC1-6) and using mouse actin beta (Actb) as housekeeping gene. (C) Representative Western Blot of GLP-1R protein expression in β-TC1 cells and in TC1-6 cells. (D) Densitometric analysis of GLP-1R protein expression in β-TC1 cells and in TC1-6 cells. The data are the means of five different experiments ± SE. (E) GLP-1R (red) immunoreactivity in TC1-6 cells. Hoechst 33258 was used to visualize nuclei (blue) and phalloidin to stain the cytoskeloton (green). Squares (upper side) indicate the areas shown at higher magnification (lower side).

Inline graphic — (A) Full length Glp1r transcript expression as analysed by RT-PCR in α-TC1-6 and in InR1G9 cells. (B) Glp1r gene expression as analysed by Real Time PCR (means of five experiments) in beta cells (β-TC1 cells as positive control) and alpha cells (TC1-6); data are expressed as (2^−ΔΔCt) considering as 1 the Glp1r gene expression in alpha cells (TC1-6) and using mouse actin beta (Actb) as housekeeping gene. (C) Representative Western Blot of GLP-1R protein expression in β-TC1 cells and in TC1-6 cells. (D) Densitometric analysis of GLP-1R protein expression in β-TC1 cells and in TC1-6 cells. The data are the means of five different experiments ± SE. (E) GLP-1R (red) immunoreactivity in TC1-6 cells. Hoechst 33258 was used to visualize nuclei (blue) and phalloidin to stain the cytoskeloton (green). Squares (upper side) indicate the areas shown at higher magnification (lower side).