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. 2014 Feb 28;9(2):e90093. doi: 10.1371/journal.pone.0090093

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© 2014 Piro et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Inline graphic — Panel (A) shows the expression of Pax 6 as determined by Real-Time PCR analysis (means from five different experiments) in control cells and in cells cultured for 72 hours in the presence of GLP-1 (100 nmol/l) alone or in combination with Exendin-9 (100 nmol/l). Panel (B) shows a representative Western Blot for the Pax6 protein expression in control cells and in cells cultured for 72 hours in the presence of GLP-1 (100 nmol/l) alone or in combination with Exendin-9 (100 nmol/l); Panel (C) shows the densitometric analysis (means from five different Western Blot experiments). The data are expressed as means ± SE. ** p<0.01, using one-way ANOVA followed by Bonferroni test. Panel (D) shows the expression of the glucagon (Gcg) gene as determined by Real-Time PCR analysis (means from five different experiments) in control cells and in cells cultured for 72 hours in the presence of GLP-1 (100 nmol/l) alone or in combination with Exendin-9 (100 nmol/l). Panel (E) shows a representative Western Blot for proglucagon protein expression in control cells and in cells cultured for 72 hours in the presence of GLP-1 (100 nmol/l) alone or in combination with Exendin-9 (100 nmol/l). Panel (F) shows the densitometric analysis (means from five different Western Blot experiments). The data are expressed as the means ± SE. * p<0.05; ** p<0.01, using one-way ANOVA followed by Bonferroni test.

Panel (A) shows the expression of Pax 6 as determined by Real-Time PCR analysis (means from five different experiments) in control cells and in cells cultured for 72 hours in the presence of GLP-1 (100 nmol/l) alone or in combination with Exendin-9 (100 nmol/l). Panel (B) shows a representative Western Blot for the Pax6 protein expression in control cells and in cells cultured for 72 hours in the presence of GLP-1 (100 nmol/l) alone or in combination with Exendin-9 (100 nmol/l); Panel (C) shows the densitometric analysis (means from five different Western Blot experiments). The data are expressed as means ± SE. ** p<0.01, using one-way ANOVA followed by Bonferroni test. Panel (D) shows the expression of the glucagon (Gcg) gene as determined by Real-Time PCR analysis (means from five different experiments) in control cells and in cells cultured for 72 hours in the presence of GLP-1 (100 nmol/l) alone or in combination with Exendin-9 (100 nmol/l). Panel (E) shows a representative Western Blot for proglucagon protein expression in control cells and in cells cultured for 72 hours in the presence of GLP-1 (100 nmol/l) alone or in combination with Exendin-9 (100 nmol/l). Panel (F) shows the densitometric analysis (means from five different Western Blot experiments). The data are expressed as the means ± SE. * p<0.05; ** p<0.01, using one-way ANOVA followed by Bonferroni test.