Table 1. Summary of the barcoded pyrosequencing data sets derived from the saeu-jeot samples and statistical analysis of microbial diversity.
Subject | Total reads | High quality reads | Average read length | OTU* | Shannon-Weaver* | Chao1* | Evenness* | |
Salt conc. | Days | |||||||
0 | 6866 | 4662 | 477 | 610 (177) | 4.9 (4.3) | 1013 (282) | 0.76 (0.82) | |
10 | 12766 | 9353 | 490 | 714 (143) | 4.3 (3.7) | 1236 (358) | 0.65 (0.74) | |
20 | 6231 | 3649 | 477 | 323 (119) | 3.8 (3.2) | 557 (227) | 0.66 (0.67) | |
30 | 2170 | 1536 | 461 | 174 (125) | 4.1 (3.8) | 293 (201) | 0.80 (0.80) | |
20% | 45 | 2903 | 2249 | 439 | 215 (95) | 4.1 (3.6) | 333 (165) | 0.76 (0.79) |
60 | 3743 | 2892 | 435 | 229 (94) | 4.1 (3.6) | 397 (147) | 0.76 (0.79) | |
80 | 6033 | 4328 | 476 | 161 (53) | 2.9 (2.0) | 251 (69) | 0.56 (0.50) | |
107 | 1712 | 1348 | 442 | 150 (107) | 3.9 (3.7) | 244 (157) | 0.78 (0.79) | |
142 | 1424 | 1129 | 432 | 131 (88) | 3.9 (3.5) | 193 (131) | 0.80 (0.78) | |
0 | 5653 | 4105 | 477 | 518 (161) | 4.8 (4.1) | 918 (251) | 0.77 (0.81) | |
10 | 2206 | 1718 | 484 | 187 (129) | 3.5 (3.3) | 295 (195) | 0.66 (0.68) | |
20 | 4817 | 3252 | 474 | 188 (88) | 3.2 (3.2) | 300 (141) | 0.61 (0.71) | |
30 | 6210 | 4564 | 473 | 280 (86) | 3.5 (3.0) | 444 (168) | 0.62 (0.67) | |
24% | 45 | 5129 | 3755 | 476 | 301 (99) | 3.8 (3.1) | 459 (135) | 0.67 (0.67) |
60 | 1732 | 1307 | 477 | 124 (94) | 3.3 (3.6) | 185 (147) | 0.68 (0.79) | |
80 | 7700 | 5418 | 453 | 521 (143) | 4.6 (3.9) | 917 (247) | 0.74 (0.78) | |
107 | 7228 | 5344 | 438 | 524 (145) | 4.8 (4.1) | 820 (226) | 0.76 (0.82) | |
142 | 4108 | 3107 | 431 | 266 (109) | 4.2 (3.6) | 373 (232) | 0.75 (0.76) | |
0 | 9769 | 6638 | 482 | 630 (172) | 5.0 (4.2) | 885 (302) | 0.77 (0.82) | |
10 | 1432 | 1101 | 481 | 216 (180) | 4.2 (4.1) | 355 (323) | 0.79 (0.80) | |
20 | 1914 | 1598 | 479 | 248 (163) | 4.3 (3.9) | 487 (279) | 0.77 (0.78) | |
30 | 1062 | 888 | 482 | 182 (169) | 4.1 (4.1) | 437 (359) | 0.79 (0.79) | |
28% | 45 | 2972 | 2480 | 492 | 238 (118) | 3.5 (3.2) | 371 (190) | 0.64 (0.68) |
60 | 959 | 844 | 500 | 74 (74) | 2.4 (2.4) | 118 (118) | 0.55 (0.55) | |
80 | 4397 | 3950 | 505 | 122 (38) | 2.2 (1.7) | 203 (53) | 0.47 (0.46) | |
107 | 2482 | 1824 | 503 | 86 (52) | 2.6 (2.4) | 110 (91) | 0.58 (0.61) | |
142 | 3408 | 2938 | 500 | 94 (51) | 2.3 (2.4) | 127 (68) | 0.50 (0.62) | |
0 | 1478 | 1047 | 484 | 134 (112) | 3.9 (3.7) | 205 (155) | 0.79 (0.79) | |
10 | 1635 | 1250 | 478 | 218 (165) | 4.4 (4.2) | 405 (307) | 0.81 (0.83) | |
20 | 2202 | 1658 | 481 | 246 (155) | 4.4 (4.2) | 468 (243) | 0.79 (0.83) | |
30 | 6191 | 4611 | 479 | 465 (152) | 4.6 (3.9) | 702 (350) | 0.75 (0.78) | |
32% | 45 | 1853 | 1542 | 488 | 301 (218) | 4.3 (4.4) | 584 (489) | 0.76 (0.81) |
60 | 3401 | 2477 | 479 | 309 (160) | 4.4 (4.0) | 451 (239) | 0.77 (0.80) | |
80 | 1195 | 1007 | 480 | 197 (165) | 4.3 (4.2) | 370 (398) | 0.82 (0.82) | |
107 | 1167 | 853 | 476 | 163 (158) | 4.1 (4.1) | 267 (247) | 0.80 (0.80) | |
142 | 2530 | 2149 | 485 | 259 (152) | 4.2 (3.8) | 423 (339) | 0.75 (0.75) |
Abbreviation: OTU, operational taxonomic unit.
* The numbers of the high quality reads were normalized to 844 reads and OUT numbers and diversity indices from the original and normalized (in parentheses) sequencing reads were calculated by the RDP pyrosequencing pipeline.