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. 2014 Jan 22;164(3):1122–1133. doi: 10.1104/pp.113.229617

Figure 2.

Figure 2.

Comparison of different chromatin isolation methods. A, E2Fa-HBH protein purification efficiency. Immunoblot analysis used an anti-His antibody to evaluate the ChIP, ChAP, and TChAP E2Fa-HBH protein purification yields. Information on the percentage of input and the estimation of the purification efficiency is given. B, ChIP, ChAP, and TChAP DNA qPCR analysis. The association of E2Fa with the proximal promoters of the well-established E2Fa target genes, ORC1b, CTF18, and ETG1, was quantified by qPCR. Enrichment of E2Fa binding was normalized using CDKA;1 and UBQ10 control promoter regions. Error bars indicate sd (n = 3).