Figure 1.

Root growth and microscopic analysis of lignin deposition of wild-type (Wt) and HCT down-regulated alfalfa. A, Overall root phenotypes of 5-week-old plants. B, Cross sections (10-μm thickness) made in the same root material presented in A, at the upper part of the root close to the stem intersection. Total lignin was determined by UV autofluorescence. All settings for these micrographs were identical. C, Closeups of roots showing a decrease in UV autofluorescence in the HCT down-regulated line. Bars in B and C = 200 μm. [See online article for color version of this figure.]