Lignin content and composition in roots of alfalfa plants grown in the field for 3 years. Lignin was measured in the parental line 208Ms-1, non-down-regulated HCT−, and down-regulated HCT+ events. The cv Regen SY4D was used as a negative control. Results are means ± sd of four biological replicates in the case of parental and negative controls and eight biological replicates for the HCT events. Values with different letters are significantly different (P < 0.05). CWR, Cell wall residue; G, guaiacyl unit; H, p-hydroxyphenyl unit; S, syringyl unit.