Figure 8.

Root nodulation of alfalfa plants grown in the field for 3 years. A, Closeups of roots showing nodules in the parental 208Ms-1 line and two independent HCT down-regulated lines (HCT3A+ and HCT30A+). Bars = 10 mm. B, Nodule number in the parental line 208Ms-1, non-down-regulated HCT−, and down-regulated HCT+ events. The cv Regen SY4D was used as a negative control. Results are means ± sd of four biological replicates in the case of parental and negative controls and eight biological replicates for the HCT events. Values with different letters are significantly different (P < 0.05). [See online article for color version of this figure.]