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. 2014 Jan 22;164(3):1175–1190. doi: 10.1104/pp.113.233866

Figure 1.

Figure 1.

Gene structure of pdNAD-MDH in the wild type and the transposon insertion line ET8629 (pdnad-mdh). A, In wild-type plants, the pdNAD-MDH gene (At3g47520) consists of one intron in the 5′ untranslated region (UTR) and one exon representing the coding sequence (CDS) followed by the 3′ UTR. Numbers represent nucleotide positions relative to the translational start +1. The position of the Ds element in the enhancer trap line pdnad-mdh and the relative position of the probe for Southern blotting are shown. Sequence and position of the target for silencing by amiRNA in the miR-mdh lines and position of restriction enzymes used in Southern blotting are indicated. Locations of primers used for genotyping are depicted as arrows. B, Southern blot analysis showed that pdnad-mdh has only one Ds insertion; 10 µg of genomic DNA per lane from wild-type (lanes 2 and 4) and heterozygous pdnad-mdh (lanes 3 and 5) plants digested with HindIII (lanes 2 and 3) or BglII (lanes 4 and 5) are shown. Position of the digoxigenin (DIG)-labeled probe on the Ds element is as shown in A. Sizes (in kilobase pairs) of the DIG-labeled molecular weight marker III (lane 1; Roche) are indicated on the left of the membrane.