Table II. Silencing of pdNAD-MDH entails reductions in photosynthetic rate.
Plants were grown under standard conditions for 3–4 weeks (wild type) and 8–9 weeks (miR-mdh-1) before transfer to gas exchange chambers (Supplemental Fig. S8, plant images). After a 2-d adaptation period, A and E were measured in custom-made gas exchange chambers over one complete 12-h-light/12-h-dark cycle at standard growth conditions. A and E were averaged over day and night, respectively. Projected leaf area was determined at the end of the light period. Values represent the mean ± se of two independent experiments with four plants per genotype each (n = 8 plants).
| A |
E |
|||
|---|---|---|---|---|
| Day | Night | Day | Night | |
| µmol m−2 s−1 | mmol m−2 s−1 | |||
| Wild type | 6.93 ± 0.25 | −0.49 ± 0.08 | 1.39 ± 0.07 | 0.96 ± 0.04 |
| miR-mdh-1 | 3.53 ± 0.12a | −0.05 ± 0.02a | 1.19 ± 0.09 | 0.91 ± 0.02 |
| Ratio miR-mdh-1:wild type (%) | 51.0 ± 1.7 | 10.7 ± 4.9 | 81.6 ± 7.0 | 91.1 ± 5.3 |
a
Values that are statistically different from the respective wild-type values (Student’s t test, P < 0.01).