Figure 3.

Wax composition is altered in gnl1-1 and ech, but not trs120-4, mutants. Quantification of cuticular wax using GC-FID reveals a significant overall change to the composition of surface waxes in gnl1-1 and ech, but not in trs120-4, relative to the wild type. In particular, gnl1-1 and ech have decreased levels of ketones and secondary alcohols (MAH1 products; green) relative to alkanes (blue). Values are means of three experiments of three biological replicates each (i.e. n = 9); error bars represent se of total surface wax load, and asterisks indicate statistically significant differences between the wild type and the mutant (Student’s t test, **P < 0.01).