Figure 6.

gnl1-1, ech, and rhd3-1 are defective in wax biosynthesis. Quantification of total waxes, including intracellular and cuticular waxes, from ground stems via GC-FID reveals that gnl1-1, ech, and rhd3-1, but not trs120-4, mutants synthesize significantly less total wax than the wild type. Values are means of three experiments of three biological replicates each (i.e. n = 9); error bars represent se of total wax load, and asterisks indicate statistically significant differences between wild-type and mutant total waxes (Student’s t test, *P < 0.05, **P < 0.01).