Figure 5.

The association of the five RBPs in Co-IPs treated with RNase. Immunoprecipitates formed using antibodies raised against RBP-A, RBP-I, RBP-J, RBP-K, or RBP-Q were treated with or without RNase and then subjected to immunoblot analysis. Protein A beads conjugated with anti-GFP were used as a negative control (GFP). Note that both RBP-A and RBP-I are found in RNase-resistant complexes with RBP-J and RBP-K but not with each other. The association of RBP-Q with RBP-A and RBP-K is RNase sensitive, indicating that the former does not directly interact with the two latter RBPs. Overall, these results support the existence of three RBP-containing multiprotein complexes.