Analysis of DEGs based on RNA-seq data. A, Distribution of 5,194 DEGs. Dashed and solid circles refer to 84021 and H05, respectively. B, Number of differentially expressed genes that were up- or down-regulated at different stages during anther development. 8N and 8H refer to 84021 under NT and HT conditions, respectively; HN and HH refer to H05 under NT and HT conditions, respectively; C, Venn diagram showing the DEGs in each of the three different anther developmental stages of the two cotton lines. The overlapping regions correspond to the number of DEGs present at more than one anther developmental stage.