Effects of the conversion of Suc and Glc on HT injury in cotton anther development. A, The expression of SUS and INV genes was validated by RT-PCR. SUS genes were SUS1 (FJ713478), SUS3 (DT571793), and SUS4 (U73587). INV genes were CWINV (ES809752), CINV(1) (ES815699), CINV(2) (ES815150), and CINV(3) (ES794929). UB7 gene was UBQUTIN7 (DQ116441). No significant differences in SUS expression and decreased INV expression were observed in H05 under HT. MS, Meiosis stage. B to D, Determination of the partitioning of total soluble sugar (B), Suc (C), and Glc (D) in 12 samples of two cotton lines under NT and HT conditions. 8N and 8H refer to 84021 under NT and HT conditions, respectively; HN and HH refer to H05 under NT and HT conditions, respectively. Data are presented as means ± se from six biologically independent experiments. Values not sharing a common letter are considered statistically significant (shortest significant range; P < 0.05).