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. 2014 Jan 30;164(3):1293–1308. doi: 10.1104/pp.113.232314

Figure 5.

Figure 5.

Effects of HT on starch synthesis and catabolism in 84021 and H05 anthers under HT and NT conditions. A, Starch content was assessed in 84021 and H05 anthers at three different developmental stages under HT and NT conditions. 8N and 8H refer to 84021 under NT and HT conditions, respectively; HN and HH refer to H05 under NT and HT conditions, respectively. B, Schematic representation and cross observation of starch staining in the anthers at ADS described in A. C, Expression levels of starch synthesis (GBSSI) and catabolism (α-AMYLASE and β-AMYLASE) genes were confirmed by qRT-PCR and RT-PCR in the anthers of 84021 and H05 under two conditions. The x axis indicates the anther developmental stages; 8 and H indicate 84021 and H05, respectively. D, The enzymatic activities of AGPase, SSSase, and GBSSase were assessed. The data in A, C, and D are presented as means ± se from three biologically independent experiments. Asterisks indicate statistically significant differences (*P < 0.05, **P < 0.01, ***P < 0.001) by Student’s t test. NS, Not significant.