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. 2014 Jan 13;164(3):1326–1337. doi: 10.1104/pp.113.228049

Figure 1.

Figure 1.

Schematic diagram of OsTrx1 and flowering phenotype of T-DNA insertional ostrx1 mutant. A, OsTrx1 has 25 exons (black boxes) and 24 introns (lines between boxes). Gray boxes indicate 5′ and 3′ untranslated regions. T-DNA shown as a triangle is located at the ninth intron (Line 2D-31249). Primers F1, R1, and GUS were used for genotyping ostrx1; F2 and R2 were used for semiquantitative RT-PCR analyses of OsTrx1 transcript levels (Supplemental Table S1). B, Semiquantitative RT-PCR analyses of OsTrx1 transcripts in ostrx1 and the segregating wild type (WT). Samples were harvested at Zeitgeber time 2 h from uppermost leaf blades of 28 DAG plants grown under LD (14-h light/10-h dark) conditions. Actin1 (LOC_Os03g50885) was used as a reference. C, Phenotypes at ripening stage for ostrx1 plants and the segregating wild type grown in the PF condition. Bar = 20 cm. D, Days to flowering of ostrx1 and the segregating wild type grown under SD (10-h light/14-h dark), LD, or PF conditions. Values are shown as means. Error bars indicate sds. n = 5 or more.